Chemical Synthesis of a Polyvinylpyrrolidone-Capped Silver Nanoparticle and Its Antimicrobial Activity against Two Multidrug-ResistantAeromonas Species

This study investigated the antimicrobial activity of polyvinylpyrrolidone-capped silver nanoparticles (PVP-AgNPs) in vitro against two antibiotic-resistant isolates Aeromonas hydrophila and A. caviae. Solid crystal were synthesized by chemical reduction approach employing polyvinylpyrrolidone (PVP) as a neutral stabilizer, glucose reducing agent, sodium hydroxide catalyst. The crystallinity purity prepared PVP-AgNPs using X-ray diffraction spectrometer (XRD). Transmission electron micrograph analysis demonstrated formation irregular spherical shapes with average crystallite size diameters ranging from 5.07 to 9.74 nm. By phenotypic gene sequence analysis, caviae identified diseased Nile tilapia. Our findings showed that all multidrug-resistant (MDR) resistance four classes. displayed 15 mm 14 clear inhibition zones for caviae, respectively. In addition, minimum inhibitory concentrations estimated be 6.25 μg/ml 7.5 chemically found possess strong effect possibility used commercialized agent aquaculture industry.